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Puromycin dihydrochloride BioChemica

Assay (HPLC): min. 98 %
Code
A2856
CAS
58-58-2
Molecular Formula
C22H29N7O5 · 2HCl
Molar mass
544.44 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A2856,0010
code
A2856,0010
packaging size
10 mg
price per unit
single 53,90€
box price per unit
A2856,0100
code
A2856,0100
packaging size
100 mg
price per unit
single 369,10€
box price per unit
Physical Description:
Solid
Product Code:
A2856
Product Name:
Puromycin dihydrochloride BioChemica
Specifications:
Assay (HPLC): min. 98 %
IR spectrum: passes test
Solubility (5 %; H2O): clear, colorless
Hazard pictograms
  • GHS07 Hazard
WGK:
1
Storage:
-20°C
Signal Word:
Attention
GHS Symbols:
GHS07
H Phrases:
H302
P Phrases:
P264
P270
P301+P312
P330
P501
EINECS:
200-387-8
CS:
29419000
Download TDS file for complete specifications

Comments

Puromycin is an aminonucleoside antibiotic from Streptomyces alboniger. It is an analogon to aminoacyl-tRNA and inhibits the protein synthesis by termination of the peptidyl transfer at the ribosomes in prokaryotes and eukaryotes. The antibiotic inhibits the growth of gram positive bacteria and different animal cells. Fungi and gram negative bacterias are resistant, since puromycin cannot pass the cell wall. The discovery of the puromycin-N-acetyltransferase (PAC) in a Streptomyces-stem allowed to employ the antibiotic as a selection marker, comparable to neomycin. The working concentration is for mammalian cells depending on the cell line between 1 and 50 μg/ml.Stock solutions of puromycin (10 mg/ml) can be prepared in HEPES buffer and are stored at \+4°C or -20°C (improves stability) up to one year.

Literature

(1) Sambrook, J. & Russell, D.W. (2001) Molecular Cloning: A Laboratory Manual, 3rd Edition Page 16.49 - 17.65. CSHL Press, Cold Spring Harbor, NY. (2) De La Luna, S. & Ortin, J. (1992) Methods Enzymol. 216, 376-385. pac-Gen as Efficient Dominant Marker and Reporter Gene in Mammalian Cells.