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CTAB - Lysis buffer BioChemica

Code
A4150

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code packaging size price per unit box price per unit
Code & packaging Price per piece
A4150,0500
code
A4150,0500
packaging size
500 ml
price per unit
single 39,80€
box price per unit
A4150,1000
code
A4150,1000
packaging size
1 L
price per unit
single 75,50€
box price per unit
Physical Description:
Liquid
Product Code:
A4150
Product Name:
CTAB - Lysis buffer BioChemica
Specifications:
pH (20°C): 8.0 ± 0.1
Composition:
CTAB: 20.00 g/L (2 % w/v)
EDTA-Na2 · 2H2O: 7.44 g/L (20 mM)
Tris: 12.11 g/L (100 mM)
Sodium chloride: 81.82 g/L (1.4 M)
Hazard pictograms
  • GHS07 Hazard
WGK:
2
Storage:
RT
Signal Word:
Attention
GHS Symbols:
GHS07
H Phrases:
H319
P Phrases:
P264
P280
P305+P351+P338
P337+P313
CS:
38221900
Download TDS file for complete specifications

Comments

The cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids. CTAB forms an insoluble complex with nucleic acids when the initial NaCl concentration is lowered to ~0.5 M. Polysaccharides, phenolic compounds and other enzyme-inhibiting contaminats found in plant cells are efficiently removed in the supernatant because most do not precipitate under these conditions (1).

Literature

(1) Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (2001) Current Protocols in Molecular Biology. Page 2.3.5 (Suppl. 45) Greene Publishing & Wiley-Interscience, New York.