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Bisacrylamide Molecular biology grade

Code
A3636
CAS
110-26-9
Molecular Formula
C7H10N2O2
Molar mass
154.17 g/mol
Physical Description:
Solid
Product Code:
A3636
Product Name:
Bisacrylamide Molecular biology grade
Specifications:
DNases/RNases/Proteases: not detectable
Purity (HPLC): min. 99 %
Free acrylic acid: max. 0.05 %
Water (K.F.): max. 0.25 %
A (1 cm/1 % in H2O)
290 nm: max. 0.25
310 nm: max. 0.05
400 nm: max. 0.005
Hazard pictograms
  • GHS06 Hazard
  • GHS08 Hazard
WGK:
2
Storage:
2 - 8°C
Signal Word:
Danger
GHS Symbols:
GHS06
GHS08
H Phrases:
H301
H341
P Phrases:
P280
P301+P310
P321
P330
P405
P501
EINECS:
203-750-9
CS:
29241900
Download TDS file for complete specifications

Comments

Polyacrylamide gels are formed by polymerization of acrylamide monomers to long chains and crosslinking of these chains with N,N'-methylenebisacrylamide (bisacrylamide). The concentration of acrylamide and the ratio of acrylamide to bisacrylamide determine the pore size of the gel. Bisacrylamide is more hydrophobic and therefore less water soluble than acrylamide. It is recommended to dissolve bisacrylamide in lukewarm water. All bisacrylamide solutions should be filtered, as is the case with acrylamide solutions.

Literature

(1) Ogden, R.C. & Adams, D.A. (1987) Methods Enzymol. 152, 61-87. Electrophoresis in agarose and acrylamide gels. (2) Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. (3) Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Greene Publishing & Wiley-Interscience, New York.