SuperHot Taq DNA Polymerase

Comments

SuperHot Taq DNA Polymerase is an optimized mixture of Taq DNA Polymerase and anti-Taq DNA polymerase monoclonal antibodies. The Antibodies block the polymerase activity during the set-up of the PCR reactions at room temperature (20 - 22°C). The inhibition of the Taq DNA polymerase is completely reversed when the temperature is above 70°C. The PCR products obtained with SuperHot Taq are free from nonspecific products and from primer-dimers. The recommended Reaction Buffer (1X) is 16 mM (NH₄)₂SO₄, 67 mM Tris · HCl (pH 8.8); 1.5 - 7 mM MgCl₂, 0.01 % Tween-20. The Reaction buffer supplied with the enzyme is a 10X concentrate of the recommended buffer. The storage buffer contains 10 mM Tris &middoz; HCl (pH 7.0); 50 mM KCl; 0.1 mM EDTA; 50 % glycerol.Recommended PCR conditions: Use the PCR conditions optimized for Taq DNA polymerase. In case of low amount of DNA template, additional cycles may be usedApplications: Complex genomic or cDNA templates, low copy number targets, large numbers of thermal cycles, multiplex PCR.Unit definition: One unit of activity is defined as the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 72°C.