Back

3,3',5,5'-Tetramethylbenzidine (TMB) BioChemica

Assay (titr.): min. 98 %
Code
A3840
CAS
54827-17-7
Molecular Formula
C16H20N2
Molar mass
240.35 g/mol

Recommended prices only. To see your prices login or contact your local distributor.
Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A3840,0001
code
A3840,0001
packaging size
1 g
price per unit
single 35,10€
box price per unit
A3840,0005
code
A3840,0005
packaging size
5 g
Product active until stock lasts.
A3840,0025
code
A3840,0025
packaging size
25 g
price per unit
single 404,10€
box price per unit
A3840,0250
code
A3840,0250
packaging size
250 g
price per unit
single 3179,00€
box price per unit
Solubility:
insoluble (H2O)
Physical Description:
Solid
Product Code:
A3840
Product Name:
3,3',5,5'-Tetramethylbenzidine (TMB) BioChemica
Specifications:
Assay (titr.): min. 98 %
Residue on ignition: max. 0.2 %
Loss on drying: max. 0.5 %
Hazard pictograms
  • GHS07 Hazard
WGK:
awg
Storage:
2 - 8°C
under argon
Signal Word:
Attention
GHS Symbols:
GHS07
H Phrases:
H315
H319
H335
P Phrases:
P261
P280
P305+P351+P338
P403+P233
P405
P501
EINECS:
259-364-6
CS:
29215990
Download TDS file for complete specifications

Comments

Tetramethylbenzidine (TMB) is used as a chromogenic substrate for the detection of horseradish peroxidase, especially applied in Enzyme Immunoassays (e. g. ELISA). It is more sensitive than o-phenylene diamine or ABTS and more stable and less toxic than diaminobenzidine. TMB has been classified as nontoxic. The color development is generally completed after 10 - 30 minutes at room temperature. The blue color turns to yellow when the reaction is stopped by the addition of 1 M sulfuric acid and the color development is measured at 450 nm.Solubility: TMB is soluble in DMSO, acetone, chloroform, toluene (100 mg/ml) or ethanol 96 % (~30 mg/ml). It is insoluble in water unless TMB forms the dihydrochloride. Stock solutions can be prepared e.g. as 0.01 M solutions (0.24 g/100 ml) up to 1.5 % (1.5 g/100 ml). Solutions are stored at 2-8°C.

Literature

(1) Volpe, G. et al. (1998) Analyst 123, 1303-1307. TMB as electrochemical substrate for horseradish peroxidase based immunoassays. A comparative study. (2) Schütz, A.J. et al. (1997) SPIE Proc. 3105, 332-340. Stabilization of Horseradish Peroxidase (HRP) for the Use in Immunochemical Sensors.