Blocking Buffer I


Recommended prices only. To see your prices login or contact your local distributor.
Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
packaging size
125 ml
price per unit
single 340,30€
box price per unit
Product Code:
Product Name:
Blocking Buffer I
• Solution for blocking unspecific binding sites in ELISA, EIA & Western blots
• Application in ELISA, EIA, RIA, Western Blot, Protein chips, Immuno-PCR and Immuno histochemistry
• efficiently blocks free protein binding sites on ELISA plates and transfer membranes
• based on chemically modifed, low-molecular weight casein
ready-to-use; stabilised with 0.1 % ProClin® 300; pH 7.2
shipment at ambient temperature
Download TDS file for complete specifications


Blocking Buffer I is based on a low-molecular weight, highly purified casein with NaCl and Tween® and stabilized with 0.1 % ProClin® 300. It does not contain glycosylated proteins. Blocking Buffer I saturates free binding capacities on plastic consumables and other surfaces like ELISA plates and blotting membranes. Thus a reduction of unspecific binding on surfaces can be achieved. Efficiency of blocking is significantly improved in comparison to standard blocking procedures by a special production method, which leads to casein molecules with many different molecular sizes. Blocking Buffer I can be used in ELISA, EIA, RIA, Western blotting, immuno-PCR, protein arrays as well as immunohistochemistry. Immediately before use the buffer should be mixed thoroughly. Blocking Buffer I is ready-to-use. Repeated freezing and thawing is possible. After immobilisation of capture antibody or target protein Blocking Buffer I is applied without dilution in wells or on membranes. Incubation time has to be adopted depending on surface characteristics by the user. We recommend blocking over night at 4°C, but in many cases shorter incubation is also promising. After blocking the surface has to be washed with Washing Buffer to make it useable for the next working steps.