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Bacitracin BioChemica

Code
A0623
CAS
1405-87-4
Molecular Formula
C66H103N17O16S
Molar mass
1422.72 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A0623,0025
code
A0623,0025
packaging size
25 g
price per unit
single $207,90
box price per unit
Physical Description:
Solid
Product Code:
A0623
Product Name:
Bacitracin BioChemica
Specifications:
Activity: min. 60 IU/mg
Solubility (1 %; H2O): clear, yellow
pH (1 %; H2O; 20°C): 6.0 - 7.0
Loss on drying: max. 5 %
Hazard pictograms
  • GHS07 Hazard
WGK:
1
Storage:
2 - 8°C
Signal Word:
Attention
GHS Symbols:
GHS07
H Phrases:
H315
H317
H319
H335
P Phrases:
P261
P280
P305+P351+P338
P403+P233
P405
P501
Origin:
from Bacillus licheniformis
EINECS:
215-786-2
CS:
29419000
Download TDS file for complete specifications

Comments

Antibiotic\: The antibiotic bacitracin from Bacillus licheniformis is composed of several peptides (A, B, C, D, E, F1-3). Bacitracin A is a cyclic dodecapeptide and with 70 % the most important component of bacitracin. For its bactericidal action, it requires divalent cations (e. g. zinc), forming stable complexes (1). It inhibits, like vancomycin, the biosynthesis of the cell wall, preferentially gram positive bacteria and cocci, by binding to bactoprenyl pyrophosphate.Protease inhibitor\: Bacitracin is employed as a protease inhibitor, too. The insulin-degrading activity of the glutathione-insulin transdehydrogenase is inhibited by 90 μM bacitracin by 50 % (2). The proline endopeptidase is inhibited by a concentration of 0.1 mM (3), the bacterial metalloendopeptidase pitrilysin by 0.5 - 0.7 mM (5). It was part of the protease inhibitor cocktail for the preparation of wheat germ extracts in a concentration of 100 μg/ml and in a reduced concentration of 10 μg/ml in the following purification steps (4).Stability\: Bacitracin is very stable as a dry substance at room temperature (moisture 55°C) it is unstable. Bacitracin is readily soluble in water or alcohols (1 mg/ml) and forms stable salts with zinc. In aqueous solutions, it is quite stable at pH 4 - 5, but will be inactivated quite fast at pH 5 - 8 at 25°C. Above pH 8, it is completely unstable. A 10 % loss of activity in solutions is observed after approx. two months.

Literature

(1)Scogin, D.A. et al. (1980) Biochemistry 19, 3348-3352Binding of nickel and zinc ions to bacitracin A. (2)Roth, R.A. (1981) Biochem. Biophys. Res. Com. 98, 431-438Bacitracin\: Inhibitor of the Insulin-degrading activity of glutathione-insulin transhydrogenase. (3)Daly, D.J. et al. (1985) Biochem. Soc. Trans. 13, 1161-1162Purification of the proline endopeptidase from human muscle. (4)Pick, L. & Hurwitz, J. (1986) J. Biol. Chem. 261, 6684-6693Purification of theRNA ligase from wheat germ. (5)Anastasi, A. et al. (1993) Biochem. J. 290, 601-607Characterisation of the bacterial metalloendopeptidase Pitrilysin.