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Bis-Tris for buffer solutions

Assay (titr.): min. 99 %
Code
A1025
CAS
6976-37-0
Molecular Formula
C8H19NO5
Molar mass
209.24 g/mol

Recommended prices only. For prices and orders please contact your local distributor.
Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A1025,0250
code
A1025,0250
packaging size
250 g
price per unit
single $316,35
box price per unit
A1025,0500
code
A1025,0500
packaging size
500 g
price per unit
single $504,75
box price per unit
A1025,1000
code
A1025,1000
packaging size
1 kg
price per unit
single $781,50
box price per unit
Physical Description:
Solid
Product Code:
A1025
Product Name:
Bis-Tris for buffer solutions
Specifications:
Assay (titr.): min. 99 %
pH (1 %; H2O): 8.8 - 9.6
Heavy metals (as Pb): max. 0.0003 %
Loss on drying: max. 1 %
Fe: max. 0.0005 %
A (1 cm/0.1 M in H2O)
280 nm: max. 0.04
340 nm: max. 0.02
WGK:
1
Storage:
RT
EINECS:
230-237-7
CS:
29221900
Download TDS file for complete specifications

Comments

Bis-Tris is an important buffer for protein and nucleic acid systems. Additionally it is used as a substitute for cacodylic acid buffer systems (4). The working concentration, mentioned in the literature (ref. 1-3), is 10 - 25 mM.

Literature

(1)Massie, B. et al. (1995) Bio/Technology 13, 602-608Improved vector for the production of HSV-ribonucleotide reductase R1 and R2. (2)Merabet, E. & Ackers, G.K. (1995) Biochemistry 34, 8554-8563Calorimetric analysis of the lcI-repressor binding to the DNA operator site. (3)Petri, V. et al. (1995) Biochemistry 34, 9977-9984Characterization of the binding of the TATA-binding factor to adenovirus E4 promoter. (4)Scopes, R.K. ed. (1994) Protein Purification (Principles and Practice) 3rd Edition, p. 352; Springer-Verlag.