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HEPES for buffer solutions

Assay (titr.): min. 99.5 %
Code
A1069
CAS
7365-45-9
Molecular Formula
C8H18N2O4S
Molar mass
238.31 g/mol

Recommended prices only. For prices and orders please contact your local distributor.
Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A1069,0100
code
A1069,0100
packaging size
100 g
price per unit
single $49,05
box price per unit
A1069,0250
code
A1069,0250
packaging size
250 g
price per unit
single $114,90
box price per unit
A1069,0500
code
A1069,0500
packaging size
500 g
price per unit
single $166,35
box price per unit
A1069,1000
code
A1069,1000
packaging size
1 kg
price per unit
single $273,60
box price per unit
$232,56x 6 units
A1069,5000
code
A1069,5000
packaging size
5 kg
price per unit
single $1180,95
box price per unit
A1069,9025BW
code
A1069,9025BW
packaging size
25 kg
price per unit box price per unit
Physical Description:
Solid
Product Code:
A1069
Product Name:
HEPES for buffer solutions
Specifications:
Assay (titr.): min. 99.5 %
pH (1 %; H2O): 4.7 - 6.0
Heavy metals (as Pb): max. 0.001 %
Water: max. 0.5 %
Chloride: max. 0.05 %
Sulfate: max. 0.05 %
WGK:
1
Storage:
RT
EINECS:
230-907-9
CS:
29335995
Download TDS file for complete specifications

Comments

Hepes is a widely used buffer in biological studies. There is only one restriction in the use of this buffer, because it interferes with the Folin protein assay. In cell culture media, it is employed as a substitute for the bicarbonate buffer at a concentration of 25 mM or as a supplement to the bicarbonate buffer (concentration 10 - 15 mM).The addition of 20 mM HEPES to TBE buffer improves the migration behaviour of certain samples in the SSCP analysis (single-strand conformation polymorphism), with a higher resolution especially if small differences in the sequences between certain bands are wanted (5).

Literature

(1) Good, N.E. et al. (1966) Biochemistry 5, 467-477. Synthesis and characterization of various 'H+ buffers' for biological research. (2) Shipman, C. (1969) Proc. Soc. Exp. Biol. Med. 130, 305-310. HEPES as a buffer in cell culture. (3) Good, N.E. & Izawa, S. (1972) Methods Enzymol. 24, 53-68. Further discussion on the selection of buffers in biological research, here with emphasis on their suitability for the study of photosynthetic processes. (4) Ferguson, W.J. et al. (1980) Anal. Biochem. 104, 300-310. Buffers to buffer hydrogen ions for biological research: synthesis of other buffers and test in cell culture, plant pathology, and virology. (5) Grady, J.K. et al. (1988) Anal. Biochem. 173, 111-115. Radicals from Good buffers. (6) Liu, Q. & Sommer, S.S. (1998) Biotechniques 25, 50-56. The SSCP phenomenon: addition of HEPES affects electrophoretic mobility.