Back

NBT BioChemica

Assay (photometr.): min. 99 %
Code
A1243
CAS
298-83-9
Molecular Formula
C40H30Cl2N10O6
Molar mass
817.65 g/mol

Recommended prices only. For prices and orders please contact your local distributor.
Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A1243,0001
code
A1243,0001
packaging size
1 g
price per unit
single $114,60
box price per unit
A1243,0005
code
A1243,0005
packaging size
5 g
price per unit
single $424,65
box price per unit
Physical Description:
Solid
Product Code:
A1243
Product Name:
NBT BioChemica
Specifications:
Assay (photometr.): min. 99 %
Solubility (1 %; hot MeOH): clear, yellow
Sulfated ash: max. 0.05 %
Water (K.F.): max. 3 %
Hazard pictograms
  • GHS07 Hazard
WGK:
1
Storage:
2 - 8°C
protected from light
Signal Word:
Attention
GHS Symbols:
GHS07
H Phrases:
H302
H315
H319
H335
P Phrases:
P261
P280
P305+P351+P338
P403+P233
P405
P501
EINECS:
206-067-4
CS:
29339980
Download TDS file for complete specifications

Comments

As a histochemical assay, glucose-6-phosphate dehydrogenase oxidizes NADPH and reduces thereby the hydrogen acceptor NBT. This results in the precipitation of colored, insoluble formazan at the site of enzyme activity (1). Store a stock solution (50 mg/ml or 75 mg/ml in 70 % aqueous DMF) at -20°C or \+4°C for up to one year (4).A completely different application of NBT is the isolation of fibrinogen from plasma samples. Therefore, plasma and NBT (1 g/L) are mixed in a ratio of 1 \: 1 and stirred for 5 - 10 minutes at room temperature. A precipitate is formed, that contains very pure fibrinogen (2).The most popular application of NBT is the determination of the activity of alkaline phosphatase in combination with BCIP (see A1117). This reaction is based on the oxidation of indoxyl to the insoluble, blue indigo by NBT (4).

Literature

(1)Negi, D.S. & Stephens, R.J. (1977) J. Histochem. Cytochem. 25, 149-154An improved method for the histochemical localization of Glucose-6 phosphate dehydrogenase in animal and plant tissue. (2)Vila, V. et al. (1984) Clin. Chim. Acta 138, 215-219Isolation of human fibrinogen using nitro blue tetrazolium (NBT). (3)Lokuta, M.A. et al. (1997) BioTechniques 22, 841-844Spectrophotometric determination of the oxidative metabolism. (4)Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Supplement 66, Page 10.8.17. Greene Publishing & Wiley-Interscience, New York. (5)Jekely, G. & Arendt, D. (2007) BioTechniques 42, 751-755Cellular resolution expression profiling using confocal detection of NBT/BCIP precipitate by reflection microscopy.