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SuperHot Taq DNA Polymerase

Code
A5231

Recommended prices only. For prices and orders please contact your local distributor.
Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A5231,0200
code
A5231,0200
packaging size
200 U
price per unit
single $291,60
box price per unit
Product Code:
A5231
Product Name:
SuperHot Taq DNA Polymerase
Specifications:
• Unit definition: see comment
• testet for Real Time PCR
• supplied with reaction buffer (10X)
Concentration: 4.5 - 5.0 U/µl
WGK:
1
Storage:
-20°C
Shipment:
wet ice
CS:
35079090
Download TDS file for complete specifications

Comments

SuperHot Taq DNA Polymerase is an optimized mixture of Taq DNA Polymerase and anti-Taq DNA polymerase monoclonal antibodies. The Antibodies block the polymerase activity during the set-up of the PCR reactions at room temperature (20 - 22°C). The inhibition of the Taq DNA polymerase is completely reversed when the temperature is above 70°C. The PCR products obtained with SuperHot Taq are free from nonspecific products and from primer-dimers. The recommended Reaction Buffer (1X) is 16 mM (NH₄)₂SO₄, 67 mM Tris · HCl (pH 8.8); 1.5 - 7 mM MgCl₂, 0.01 % Tween-20. The Reaction buffer supplied with the enzyme is a 10X concentrate of the recommended buffer. The storage buffer contains 10 mM Tris &middoz; HCl (pH 7.0); 50 mM KCl; 0.1 mM EDTA; 50 % glycerol.Recommended PCR conditions: Use the PCR conditions optimized for Taq DNA polymerase. In case of low amount of DNA template, additional cycles may be usedApplications: Complex genomic or cDNA templates, low copy number targets, large numbers of thermal cycles, multiplex PCR.Unit definition: One unit of activity is defined as the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 72°C.