Back

ABTS® BioChemica

Code
A1088
CAS
30931-67-0
Molecular Formula
C18H24N6O6S4
Molar mass
548.69 g/mol

Recommended prices only. For prices and orders please contact your local distributor.
Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A1088,0001
code
A1088,0001
packaging size
1 g
price per unit
single $70,65
box price per unit
A1088,0005
code
A1088,0005
packaging size
5 g
price per unit
single $263,40
box price per unit
A1088,0010
code
A1088,0010
packaging size
10 g
price per unit
single $433,35
box price per unit
Physical Description:
Solid
Product Code:
A1088
Product Name:
ABTS® BioChemica
Headline Comment:
® registered trademark of Roche Diagnostics
Specifications:
TLC: passes test
E 0.001 %/1 cm, 340 nm: min. 38000
Solubility (2 %; H2O): clear, green
Loss on drying: max. 6 %
Hazard pictograms
  • GHS07 Hazard
WGK:
1
Storage:
2 - 8°C
Signal Word:
Attention
GHS Symbols:
GHS07
H Phrases:
H315
H319
H335
P Phrases:
P305+P351+P338
EINECS:
250-396-6
CS:
29339980
Download TDS file for complete specifications

Comments

At concentrations above 56 mM, ABTS is insoluble. Heating facilitates dissolution of ABTS, e. g. in 0.1 M phosphate buffer. If it crystallizes upon cooling (e. g. at concentrations of 1 mg/ml), the supernatant can be used. This behavior can be used to separate excess of ABTS (2). At concentrations below 0.002 mM, it may happen, that, depending on the peroxidase concentration, the substrate ABTS will be completely used up and measurements of enzyme activity will be carried out in the non-linear range. Absorption maxima of ABTS are found at 340 nm and 414 nm, respectively. Usually, measurements will be performed at 420 nm.Solutions of ABTS in 0.5 M phosphate-citrate buffer (pH 4.0) are stable for at least one month, if stored at \+4°C (5).

Literature

(1)Childs, R.E. & Bardsley, W.G. (1975) Biochem. J. 145, 93-103The steady-state kinetics of peroxidase with ABTS as chromogen. (2)Bruss, M.L. & Black, A.L. (1978) Anal. Biochem. 84, 309-312Enzymatic micro determination of glycogen. (3)Gallati, H. (1979) J. Clin. Chem. Clin. Biochem. 17, 1-7Horseradish peroxidase\: determination of the activity with H₂O₂ and ABTS. (4)Mäkinen, K.K. & Tenovuo, J. (1982) Anal. Biochem. 126, 100-108Observations on the use of guaiacol and ABTS as Peroxidase substrates. (5)Szutowicz, A. et al. (1984) Anal. Biochem. 138, 86-94Colorimetric determination of monoaminoxidase with ABTS and Peroxidase.