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D(+)-Biotin BioChemica

Assay (titr.): min. 99 %
Code
A0969
CAS
58-85-5
Molecular Formula
C10H16N2O3S
Molar mass
244.31 g/mol

Recommended prices only. For prices and orders please contact your local distributor.
Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A0969,0001
code
A0969,0001
packaging size
1 g
price per unit
single $85,95
box price per unit
molecule for: D(+)-Biotin BioChemica
Solubility:
0.2 g/L (H2O)
Physical Description:
Solid
Product Code:
A0969
Product Name:
D(+)-Biotin BioChemica
Specifications:
Assay (titr.): min. 99 %
α20°C/D; 1 %, 0.1 N NaOH: +89° - +93°
Heavy metals (as Pb): max. 0.001 %
Loss on drying: max. 1 %
WGK:
1
Storage:
2 - 8°C
EINECS:
200-399-3
CS:
29362900
Download TDS file for complete specifications

Comments

The highly specific affinity of Biotin to Avidin is used for the purification of Avidin from albumins. For affinity chromatography Biotin is often fixed on a Sepharose matrix with a Poly-L-Lysin spacer (ref. 2). The Biotin Avidin (Streptavidin) system has many advantages for the purification of factors, if one of the components is coupled to the factor.Biotin is soluble in hot water, diluted acids and bases. It is insoluble in cold water, ethanol, ether and chloroform.

Literature

(1)Knappe, J. (1970) Ann. Rev. Biochem. 39, 757-776Review article\: Mechanism of Biotin action. (2)Bayer, E. & Wilchek, M. (1974) Methods Enzymol. 34 B, 265-267Insolubilized Biotin for the purification of Avidin.