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Doxycycline hyclate BioChemica

Assay (HPLC, calc. on H2O and EtOH free subst.): min. 95 %
Code
A2951
CAS
24390-14-5
Molecular Formula
C22H25ClN2O8·½H2O·½C2H6O
Molar mass
512.94 g/mol

Recommended prices only. For prices and orders please contact your local distributor.
Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A2951,0025
code
A2951,0025
packaging size
25 g
price per unit
single $226,65
box price per unit
Physical Description:
Solid
Product Code:
A2951
Product Name:
Doxycycline hyclate BioChemica
Specifications:
Assay (HPLC, calc. on H2O and EtOH free subst.): min. 95 %
Solubility (1 %; H2O): clear, yellow-green
Ethanol: max. 7 %
Water: max. 5 %
Hazard pictograms
  • GHS07 Hazard
  • GHS08 Hazard
WGK:
1
Storage:
2 - 8°C
protected from light
Signal Word:
Attention
GHS Symbols:
GHS07
GHS08
H Phrases:
H302+H332
H315
H319
H335
H361
H362
P Phrases:
P260
P263
P280
P305+P351+P338
P405
P501
EINECS:
234-198-7
CS:
29413000
Download TDS file for complete specifications

Comments

Doxycycline is a member of the Tetracycline family of antibiotics and is active against grampositive and gramnegative germs. Accordingly, it shows the same mechanism of action, i.e. an inhibition of the protein synthesis at the ribosomes. In the TET expression system, Doxycycline may replace Tetracycline. The working concentration for the inactivation of tTA in double-stable cell lines is 1 - 20 ng/ml in the culture medium (acc. to ref. 1). Solubility and stability: The crystalline substance of Doxycycline is stable. The hyclate salt of Doxycycline is watersoluble. Stability of Doxycylcine in aqueous solutions depends on the pH value: an acidic pH value increases stability (more than 30 days at ambient temperature without loss of activity at pH 1 - 2,5). At pH 7 and ambient temperature, it is stable for more than 1 week. Doxycycline forms complexes with calcium ions, which leads to an inactivation of the antibiotic. Avoid high calcium concentrations in your assay.

Literature

(1) Sambrook, J. & Russell, D.W. (2001) Molecular Cloning: A Laboratory Manual, 3rd Edition. Page 17.59. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.