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Ficoll® 400 BioChemica

Code
A2252
CAS
26873-85-8
Molar mass
approx. 400000 g/mol

Recommended prices only. For prices and orders please contact your local distributor.
Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A2252,9025
code
A2252,9025
packaging size
25 kg
price per unit
single Request a quote
box price per unit
Physical Description:
Solid
Product Code:
A2252
Product Name:
Ficoll® 400 BioChemica
Short Description:
additional product description: non-ionic, synthetic polymere of sucrose
Headline Comment:
® registered trademark Pharmacia Biotech
Specifications:
Solubility (10 %; H2O): clear, colorless
Heavy metals (as Pb): max. 0.001 %
Loss on drying: max. 3 %
WGK:
1
Storage:
RT
EINECS:
200-334-9
CS:
39072920
Download TDS file for complete specifications

Comments

Ficoll® 400 is a component of Denhardt's solution and several gel loading buffers (2 [Page 6.12]). The addition of Ficoll® 400, sucrose or glycerol increase the density of the loading buffers and facilitate the loading of the sample onto the gel. Composition of loading buffers: I. 0.25 % Bromophenol blue, 0.25 % xylene cyanol FF, 15 % Ficoll® 400 in water (store at room temperature)(according to ref. 2) II. Alkaline loading buffer 300 mM NaOH, 6 mM EDTA, 18 % Ficoll® 400, 0.15 % Bromocresol green, 0.25 % xylene cyanol FF in water (store at +4°C)

Literature

(1) Denhardt, D.T. (1966) Biochem. Biophys. Res. Com. 23, 641-646. A membrane-filter technique for the detection of complementary DNA. (2) Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition; p. 9.47-9.55, 6.12. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.