Spermine belongs to the Polyamines. When precipitating selectivly DNA with the help of Spermine most of the proteins and triphosphates will not coprecipitate. The precipitation with spermine is applied after restriction digests, the separation of DNA fragments and triphosphates and the purification of DNA fragments after PAGE. Stock solutions are prepared with a concentration of 100mM (pH 6.8, ref. 2) and stored at +4°C (short-term) or at -20°C (long-term). The working concentration is approx 0.1 mM, if the DNA is dissolved in TE.
(1) Blumenthal, A.B. et al. (1979) J. Cell Biol. 81, 255-259. Rapid isolation of Metaphase chromosomes containing high molecular weight DNA. (2) Hoopes, B. & McClure, W. (1981) Nucleic Acids Res. 9, 5493-5504. Studies on the selectivity of DNA precipitation by spermine. (3) Tabor, C.W. & Tabor, H. (1984) Ann. Rev. Biochem. 53, 749-790. Review article: Polyamines. (4) Cram, L.S. et al. (1990) Methods Cell Biol. 33, 377-382. Polyamine buffer for bivariate human flow cytogenetic analysis and sorting.