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Tetracycline hydrochloride

Assay (HPLC): min. 95 %
Code
A2228
CAS
64-75-5
Molecular Formula
C22H24N2O8 · HCl
Molar mass
480.90 g/mol

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Box prices only valid with purchase of full box.

code packaging size price per unit box price per unit
Code & packaging Price per piece
A2228,0025
code
A2228,0025
packaging size
25 g
price per unit
single $84,30
box price per unit
Physical Description:
Solid
Product Code:
A2228
Product Name:
Tetracycline hydrochloride
Specifications:
Assay (HPLC): min. 95 %
α20°C/D; 0.5 %, 0.1 M HCl, calc. on dried subst.: -240° - -255°
pH (1 %; H2O): 2.0 - 3.0
Loss on drying (3 h; 60°C; vacuum): max. 2 %
Hazard pictograms
  • GHS07 Hazard
  • GHS08 Hazard
WGK:
1
Storage:
RT
protected from light
Signal Word:
Attention
GHS Symbols:
GHS07
GHS08
H Phrases:
H315
H319
H335
H361d
H362
P Phrases:
P260
P263
P280
P305+P351+P338
P405
P501
EINECS:
200-593-8
CS:
29413000
Download TDS file for complete specifications

Comments

Tetracycline was prepared first syntheticaly from chlorotetracycline, later it was isolated from Streptomyces viridifaciens. Tetracycline is active against gram positive and gram negative cocci and bacteria. The introduction of the tetracycline-controlled gene expression system brought a new application for the antibiotic molecular biology (2-4).Stability: Tetracycline hydrochloride is stable if stored in a dry place and protected from light. It is water-soluble with approx. 11 mg/ml at 28°C. The stability in solutions is optimal at pH 3 - 5.2 (shelf life approx. 6 - 12 days). It may be stored between -20°C and 37°C. Since Tetracyclin is light-sensitive, solutions and agar plates containing TC should be protected from light. The recommended working concentration is 10 - 50 μg/ml, the stock solution e.g. 1.25 mg/ml. Magnesium ions antagonize with the activity of TC.

Literature

(1) Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. (2) Gossen, M. & Bujard, H. (1992) Proc. Natl. Acad. Sci. USA 89, 5547-5551. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. (3) Gossen, M. et al. (1995) Science 268, 1766-1769. Transcriptional activation by Tetracyclines in mammalian cells. (4) Yin, D.X. et al. (1996) Anal. Biochem. 235, 195-201. Tetracycline-controlled gene expression system achieves high-level and quantitative control of gene expression. (5) Freundlieb, S. et al. (1997) Methods Enzymol. 283, 159-173. Tetracyclin-controlled gene expression systems for investigations of the cell cycle.