A well-known inductor of protein expression in transgenic models is IPTG. We offer different qualities and achieve high customer satisfaction at good prices
One of the most important supplemental chemicals you need for Protein Biochemistry work is Albumin. Our customer favourites are:
| Code | |
| A4344 | Albumin crude from chicken egg |
| A2245 | Albumin from chicken egg |
| A0850 | Albumin (BSA) EIA and RIA grade |
| A2244 | Albumin (BSA) Fraction V (pH 5.2) |
| A1391 | Albumin (BSA) Fraction V (pH 7.0) |
| A6588 | Albumin (BSA) Fraction V (pH 7.0) for Western blotting |
| A0849 | Albumin (BSA) for microbiology |
When working with proteins, proteindegradation and digestion is an issue. ITW Reagents offers an interesting range of products to impede this to happen. We also have products for basic protein assays and detection. One of our customer favorites is Coomassie® Brilliant Blue (A3480, A1092).
Protease Inhibitors - Survey
| Prod.-No. | Description | Inhibitor Class | Target Enzyme | Recommended Working Concentration | Stock Solution |
| A1421 | AEBSF | Serine proteases | Thrombin, Chymotrypsin, Kallikrein, Plasmin, Proteinase K, Trypsin | 0.1 - 2 mM | 20 - 100 mM in buffer |
| A2266 | epsilon-Aminocaproic acid | Serine proteases | 5 mM | 500 mM in buffer | |
| A2129 | Antipain | Serine/Cysteine proteases | Trypsin, Papain, Cathepsin B | 10 - 50 µg/ml | 10 mg/ml in water, DMSO, MeOH |
| A2132 | Aprotinin | Serine proteases | Trypsin, Chymotrypsin, Kallikrein, Plasmin | 2 - 10 µg/ml | 10 mg/ml in water |
| A2144 | Chymostatin | Serine/Cysteine proteases | α-, β-, γ-, δ-Chymotrypsin, Papain, Cathepsin A, B and D | 6 - 60 µg/ml | 20 mg/ml in DMSO, acetic acid |
| (10 - 100 µM) | |||||
| A2157 | E-64 | Cysteine proteases | Papain, Bromelain, Calpain, Cathepsin B, H, L, Tumor-Cathepsin, Streptococcus-Protease, Ficin | 10 µM | 1 - 10 mM in DMSO, 50 % EtOH |
| A0878 | EGTA | Metallo proteases | KEX 2, Calcium-dependent proteases | 1 - 10 mM | in aqueous solution |
| A1666 | Iodoacetamide | Serine proteases | Cerastocytin | 1 - 5 mM | in aqueous solution |
| (185 - 925 µg/ml) | |||||
| A2183 | Leupeptin | Serine/Cysteine proteases | Plasmin, Trypsin, Papain, Cathepsin B, Thrombin, Calpain | 5 - 50 µg/ml | 1 mg/ml in aqueous solutions |
| (10 - 100 µM) | |||||
| A2205 | Pepstatin A | acidic proteases, Aspartic proteases | Pepsin, Cathepsin D, Renin, HIV- and MMTV-Protease | 1 - 5 µM | in MeOH, EtOH, acetic acid, DMSO |
| (0.7 - 3.5 µg/ml) | |||||
| A0999 | PMSF * | Serine/Cysteine proteases | Trypsin, Chymotrypsin, Thrombin, Papain | 0.1 - 1 mM | 10 - 100 mM in EtOH |
| (1.74 - 17.4 mg/ml) | |||||
| A1799 | TLCK | Serine proteases | Trypsin, Cerastocytin, not Chymotrypsin | 50 µg/ml | 1 - 20 mg/ml in buffer |
| pH <= 6 |
* The inhibition of cysteine proteases can be reverted by reducing thioles (e.g. DTT).
Abbreviations
AEBSF (4-(2-Aminoethyl)-benzolsulfonylfluoride); APMSF (4-Amidinophenylmethanesulfonylfluoride); E-64 (N-(trans-Epoxysuccinyl)-L-leucine-4-guanidinobutylamide); EDTA (Ethylenediaminetetraacetate); EGTA (Ethyleneglycol-bis-(2-aminoethyl)-tetraacetate); EtOH (Ethanol); MeOH (Methanol)PMSF (Phenylmethanesulfonylfluoride); TLCK (Nα-Tosyl-L-lysine chloromethylketone).
