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TRItidy G™

Code
A4051

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Box prices only valid with purchase of full box.

Code packaging size price per unit quantity
Code & packaging Price per piece
A4051,0100
Code
A4051,0100
packaging size
100 ml
price per unit
single 100,80€
quantity
A4051,0200
Code
A4051,0200
packaging size
200 ml
price per unit
single 191,40€
quantity
Physical Description:
Liquid
Product Code:
A4051
Product Name:
TRItidy G™
Short Description:
additional product description: Ready-to-use - solution for simultaneous isolation of RNA, DNA and proteins
Headline Comment:
* TRItidy G™ is a registered trademark of AppliChem GmbH.
Specifications:
• mono-phasic reagent (contains phenol and guanidinium thiocyanate)
• suited for small and large samples
• for samples of human, animal, plant and bacterial origin
• isolation of intact total RNA from tissue and cells, sequential precipitation of DNA and protein
• improved version of the 'single-step' RNA-isolation method developed by Chomczynski & Sacchi
• isolation of large and small RNA-species (0.1 - 15 kb) with high purity
Hazard pictograms
  • GHS05 Hazard
  • GHS06 Hazard
  • GHS08 Hazard
UN:
2821
Class/PG:
6.1/II
ADR:
6.1/II
IMDG:
6.1/II
IATA:
6.1/II
WGK:
2
Storage:
2 - 8°C
protected from light
under argon
Signal Word:
Danger
GHS Symbols:
GHS05
GHS06
GHS08
H Phrases:
EUH032
EUH208
H302+H312
H314
H330
H341
H373
H412
P Phrases:
P280
P303+P361+P353
P305+P351+P338
P310
P320
P362+P364
P405
P501
CS:
38221900
Download TDS file for complete specifications

Comments

The simultaneous isolation of RNA, DNA and proteins from biological samples has been introduced by Chomczynski (1) and improved by others. Chomczynski's method is based on the isolation of non-degraded nucleic acids (RNA and DNA) with guanidinium salts and phenol (e. g. ref. 2-5).TRItidy G™ is a reagent, based on the Chomczynski method (1), with additional modifications to improve the purity of the RNA, DNA and proteins. First, the RNA will be selectively retained in the aqueous phase during the acidic GuaSCN/Phenol extraction, while DNA and proteins stay in the organic phase and interphase, respectively (6). The DNA is isolated from the interphase/organic phase by a simple ethanol precipitation and proteins can be isolated from the remaining organic phase.

Literature

(1) Chomczynski, P. (1993) BioTechniques 15, 532-537A single-step purification method for RNA, DNA and proteins from cell and tissue samples. (2) Cox, R.A. (1968) Methods Enzymol. 12 Part B, [103a] 120-129. The use of guanidinium chloride in the isolation of nucleic acids. (3) Kirby, K.S. (1968) Methods Enzymol. 12 Part B, [98] 87-99. Isolation of nucleic acids with phenolic solvents. (4) MacDonald, R.J. et al. (1987) Methods Enzymol. 152, 219-227. Isolation of RNA with guanidinium salts. (5) Feramisco, J.R. et al. (1982) J. Biol. Chem. 257, 11024-11031. Modification of the GTC-Method for the RNA isolation according to Chirgwin. (6) Wallace, D.M. (1987) Methods Enzymol. 152, 33-41. Large and small scale phenol extraction. (7) Chomczynski, P. & Mackey, K. (1995) Anal. Biochem. 225, 163-164. Substitution of chloroform by bromochloropropane in the Single-step method of RNA-isolation (8) Chomczynski, P. & Mackey, K. (1995) BioTechniques 19, 942-945. Modification of the TRI Reagent procedure for isolation of RNA from polysaccharide- and proteoglycan-rich sources. (9) Monstein, H.-J. et al. (1995) BioTechniques 19, 340-344. RNA extraction from gastrointestinal tract and pancreas by a modified Chomczynski and Sacchi method.