ITW Reagents offers a broad spectrum of antibiotics and antimycotics for use in cell culture and as media supplement.
As an alternative to classical cell culture antibiotics like penicillin-streptomycin, ITW Reagents provides a product to prevent microbial growth in cell cultures: CellCultureGuard (A8906,0050). This combination of selected antibiotics (one being a fluoroquinolone) offers a wide range of anti-microbial activity, making it our first choice cell culture reagent: CellCultureGuard is active against extra- and intracellular bacteria, mycoplasma, protozoa and fungi (yeast). Additionally, it is highly compatible with resistance markers and bears a low risk of resistance development.
| Product-No. | Description | Target organism | Mode of action | recommended working concentration | Stock solution |
| A1489 | Actinomycin D | cytotoxic, anti-neoplastic | Peptide antibiotic; binds to GC base pairs of the DNA (intercalator), blocks transcription mediated by RNA polymerase | 0.04-50 µg/ml | 5 mg/ml in MeOH/EtOH at +4°C |
| A1907 | Amphotericin B | fungi, yeast | binds to sterols with planar structure and impairs with membrane permeability | 0.25 µg/ml | 30-40 mg/ml in DMSO |
| >3 µg/ml fungicidal | |||||
| A0839 | Ampicillin sodium salt | gram positive/ | inhibits cell wall synthesis (transpeptidase) in growing bacteria | 20-60 µg/ml | 50 mg/ml in H2O |
| negative bacteria and cocci | atore at -20°C | ||||
| A0623 | Bacitracin | gram positive bacteria and cocci | inhibits cell wall synthesis (peptidoglycan synthesis) by binding to bactoprenyl pyrophosphate; also employed as a general protease inhibitor | 1 mg/ml | up to 1 g/ml in H2O or alcohols |
| A3784 | Blasticidin S hydrochloride | Prokaryotes, Eukaryotes | inhibits protein biosynthesis by preventing the formation of the peptide bond | 3-100 µg/ml | 50 mg/ml in H2O or buffer store at +4°C - -20°C |
| A2138 | Brefeldin A | yeasts, gram negative bacteria, eukaryotes, viruses | Lactone antibiotic; inhibits the intracellular protein transport and the secretion of proteins; destruction of Golgi apparatus | 1-5 µM or 0.1-10 µg/ml | 1-20 mM or 10 mg/ml in MeOH bei -20°C lagern |
| A1491 | Carbenicillin disodium salt | gram negative germs, enterococci | inhibits cell wall synthesis (transpeptidase) in growing bacteria | 20-60 µg/ml | 50 mg/ml in H2O/MeOH 1:1 store at -20°C |
| A1806 | Chloramphenicol | gram positive cocci and bacteria, | binds to 50 S ribosomal subunit of prokaryotes; inhibits formation of peptide bonds | 20-170 µg/ml | 10 or 34 mg/ml in EtOH |
| gram negative bacteria | store at -20°C | ||||
| A3785 | Chlorhexidine diacetate · H2O | mycobactericidal, fungicidal, bactericidal | interaction with components of the cell membrane | 10-100 µg/ml bacteria; 100-1000 µg/ml yeasts | up to 1.9 g/100 ml H2O |
| A2922 | Colistin sulfate | gram negative bacteria - non- proliferating germs too | interaction with phospholipid components of the bacterial cell membrane; modifies permeability of the membrane and leads to an efflux of essential plasma substances | 0.5-100 µg/ml | > 1 mg/ml water-soluble |
| A0879 | Cycloheximide (Actidion) | fungi, eukaryotes | binds to 80 S ribosome in eukaryotic cells; inhibits formation of peptide bond | 10 µg/ml | 10 mg/ml |
| A1943 | D-Cycloserine | gram positive, gram negative bacteria; various protozoa, spirochaeta, rickettsia | competitive antagonist of D-alanine; Inhibition of the enzymes alanine-racemase, alanine-synthetase and several Transaminases; causes cell wall damages. | 200 µg/ml | 10-100 mg/ml in H2O or 0.1 M sodium phosphate buffer pH 8 (more stable at alkaline pH!); recommendation: prepare fresh immediately before use! |
| A2167 | G418 disulfate | toxic to bacteria, yeast higher plants, protozoa, mammalian cells | Aminoglycoside Antibiotic; | 50-1000 µg/ml | 2 mg/ml in H2O or medium; adjust pH to 7.4; store at +4°C |
| (frequently 0.4-1 mg/ml) | |||||
| A1492 | Gentamycin sulfate | gram positive/ negative germs | inhibits protein synthesis by binding to the L6 protein of the 50 S ribosomal subunit | 15-50 µg/ml | 10-20 mg/ml in water, formamide |
| A5347 | Hygromycin B | Mycoplasma, eukaryotic and prokaryotic cells | inhibits the protein synthesis by termination of the translocation and causes mistakes in transcription | 10-400 µg/ml | 400 mg/ml in H2O or buffer; store at -20°C |
| A2175 | Hygromycin B - Solution | 10-400 µg/ml | ca. 41 mg/ml in H2O; store at -20°C | ||
| A1493 | Kanamycin sulfate | gram positive/ | inhibits protein synthesis (translocation) | 10-100 µg/ml | 10 mg/ml in H2O |
| negative cocci & bacteria | store at -20°C | ||||
| A2190 | Mitomycin C | gram positive bacteria, some viruses, cytostatic | covalent binding to DNA, cross-linking of complementary DNA strands; leads to strand breakes | 10 µg/ml | 0.2 mg/ml in PBS, sterilize by filtration; store up to 6 months at -20°C |
| A1894 | Nalidixic acid | gram negative cocci and bacteria | inhibits specifically DNA replication in prokaryotes (DNA gyrase) | 15 µg/ml | CHCl3, Toluene, |
| 5-10 mg/ml | |||||
| in 0.1 N NaOH (pH 11) | |||||
| A1944 | Novobiocin sodium salt | gram positive bacteria and cocci, gram negative cocci | inhibition of initiation of DNA synthesis, competes with ATP binding at the DNA gyrase | 1-5 µg/ml | 100 mg/ml H2O (pH 7.5) |
| A3811 | Nystatin dihydrate | yeast, fungi | Polyen Macrolide; interacts with sterols from cell membrane; changes permeability | 20-50 µg/ml | ‹100 mg/ml H2O, readily soluble in DMF and DMSO |
| A1837 | Penicillin G potassium salt | gram positive bacteria and cocci, gram negative cocci, Actinomycetes, Spirochaetae | irreversible interaction with the transpeptidase; inhibits cell wall synthesis | 50-100 µg/ml | H2O |
| or 20-50 U/ml | |||||
| A0890 | Polymyxin B sulfate | gram negative bacteria - non- proliferating germs too | interaction with phospholipid components of the bact. cell membrane; changes permeability of the membrane and causes efflux of essential plasma compounds | 50 µg/ml | 25 mg/ml H2O, MeOH |
| A2856 | Puromycin dihydrochloride | gram positive bacteria; several Eukaryotes | analogon of the terminal amino acyladenosine part of tyrosyl-tRNA; binds instead of amino acyl-tRNA to ribosomes; termination of protein biosynthesis; | 10-100 µM | 100 mM in H2O; store at -20°C |
| A2220 | Rifampicin | Prokaryotes, Eukaryotes (high dosis) | inhibits RNA synthesis by binding to the b-subunit of the RNA polymerase | 150 µg/ml | 34 mg/ml in DMSO, MeOH |
| A3834 | Spectinomycin · 2HCl · 5H2O | aerobic gram-negative, -positive bacteria and mycoplasma species | Aminoglycoside antibiotic; bacteriostatic; inhibits translocation of peptidyl tRNA from the A site to the P site | 100 µg/ml | 10 mg/ml in H2O, MeOH, DMSO |
| A1852 | Streptomycin sulfate | mycobacteria, gram negative/ positive cocci and bacteria | inhibits protein synthesis by binding to the 30 S ribosomal subunit; causes missreading of the DNA code | 10-100 µg/ml | 10-50 mg/ml in H2O store at -20°C |
| A2228 | Tetracyclin hydrochloride | 12 µg/ml | approx. 12 mg/ml in H2O | ||
| A2242 | Tunicamycin | gram positive bacteria, yeasts, fungi, viruses | inhibits the GlcNAc-1P transferase (linking fatty acids : saccharides) | 0.1-5 µg/ml | 10 µg/ml in 0.05 M NaOH store at -20°C |
| A1839 | Vancomycin hydrochloride | bacteriostatic and bacterizide against gram positive cocci and bacteria | amphoteric glyopeptide antibiotic; binds to bacterial cell wall precursors (peptidoglycans) | 1-25 µg/ml | soluble in water ›100 mg/ml |