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Lysozyme BioChemica

Code
A3711
CAS
9001-63-2
Masse molaire
approx. 14400 g/mol

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code format d’emballage prix par unité prix de boîte par unité
Code et emballage Prix par pièce
A3711,0001
code
A3711,0001
format d’emballage
1 g
prix par unité
simple 40,80€
prix de boîte par unité
A3711,0010
code
A3711,0010
format d’emballage
10 g
prix par unité
simple 196,00€
prix de boîte par unité
A3711,0050
code
A3711,0050
format d’emballage
50 g
prix par unité
simple 725,50€
prix de boîte par unité
Physical Description:
Solid
Product Code:
A3711
Product Name:
Lysozyme BioChemica
Short Description:
additional product description: Hydrochloride form salt-free, albumin-free, freeze-dried
Specifications:
Activity (pH 6.2): min. 20000 U/mg
WGK:
1
Storage:
-20°C
Shipment:
RT
Origin:
from chicken egg white
EINECS:
232-620-4
CS:
35079090
Télécharger le fichier TDS pour les spécifications complètes

Literature

Lysozyme (muramidase) preferentially hydrolyzes the β-1,4-glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine, components of the proteoglycan cell wall of certain microorganisms. This enzyme is found in many organisms. In molecular biology, the enzyme from chicken egg white is commonly used for lysis of E. coli to isolate plasmids (Ref. 1 Mini-Prep: Suppl. 15; pp. 1.6.4-7). The working concentration is 200 μg/300 μl. In the so-called "Maxi-Prep", lysozyme can also be added to increase (approx. 5 - 10%) the plasmid yield (Ref. 1 Maxi-Prep: Suppl. 41; pp. 1.7.2-4). Another application is the lysis of bacteria for the preparation of bacterial RNA (Ref. 1 Suppl. 15; pp. 4.4.4-5). In this case, the working concentration is 40 μg/ml (stock solution 50 mg/ml). Form: The protein itself is lysozyme chloride, the chloride is part of the protein structure. The preparation does not contain free sodium chloride. The maximum chloride content is 4%. Stability: Lyophilized lysozyme powder is stable at +4°C for many years. In solution, stability is several weeks at pH values of 4 - 5 at +4°C and several days at room temperature. The optimum pH is 9.2, the isoelectric point is 11.0. Lysozyme is inhibited by surfactants such as SDS or alcohols and fatty acids, imidazole and indole derivatives. Stock solutions in 10 mM Tris-Cl (pH 8.0) are usually prepared at a concentration of 10, 25 or 50 mg/ml, usually just before use.