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Antibiotics & Antimycotics

ITW Reagents offers a broad spectrum of antibiotics and antimycotics for use in cell culture and as media supplement.


As an alternative to classical cell culture antibiotics like penicillin-streptomycin, ITW Reagents provides a product to prevent microbial growth in cell cultures: CellCultureGuard (A8906,0050). This combination of selected antibiotics (one being a fluoroquinolone) offers a wide range of anti-microbial activity, making it our first choice cell culture reagent: CellCultureGuard is active against extra- and intracellular bacteria, mycoplasma, protozoa and fungi (yeast). Additionally, it is highly compatible with resistance markers and bears a low risk of resistance development.

Product-No. Description Target organism Mode of action recommended working concentration Stock solution
A1489 Actinomycin D cytotoxic, anti-neoplastic Peptide antibiotic; binds to GC base pairs of the DNA (intercalator), blocks transcription mediated by RNA polymerase 0.04-50 µg/ml 5 mg/ml in MeOH/EtOH at +4°C
A1907 Amphotericin B fungi, yeast binds to sterols with planar structure and impairs with membrane permeability 0.25 µg/ml 30-40 mg/ml in DMSO
>3 µg/ml fungicidal
A0839 Ampicillin sodium salt gram positive/ inhibits cell wall synthesis (transpeptidase) in growing bacteria 20-60 µg/ml 50 mg/ml in H2O
negative bacteria and cocci store at -20°C
A0623 Bacitracin gram positive bacteria and cocci inhibits cell wall synthesis (peptidoglycan synthesis) by binding to bactoprenyl pyrophosphate; also employed as a general protease inhibitor 1 mg/ml up to 1 g/ml in H2O or alcohols
A3784 Blasticidin S hydrochloride Prokaryotes, Eukaryotes inhibits protein biosynthesis by preventing the formation of the peptide bond 3-100 µg/ml 50 mg/ml in H2O or buffer; store at +4°C - -20°C
A2138 Brefeldin A yeasts, gram negative bacteria, eukaryotes, viruses Lactone antibiotic; inhibits the intracellular protein transport and the secretion of proteins; destruction of Golgi apparatus 1-5 µM or 0.1-10 µg/ml 1-20 mM or 10 mg/ml in MeOH; store at -20°C
A1491 Carbenicillin disodium salt gram negative germs, enterococci inhibits cell wall synthesis (transpeptidase) in growing bacteria 20-60 µg/ml 50 mg/ml in H2O/MeOH 1:1 store at -20°C
A1806 Chloramphenicol gram positive cocci and bacteria, binds to 50 S ribosomal subunit of prokaryotes; inhibits formation of peptide bonds 20-170 µg/ml 10 or 34 mg/ml in EtOH
gram negative bacteria store at -20°C
A3785 Chlorhexidine diacetate · H2O mycobactericidal, fungicidal, bactericidal interaction with components of the cell membrane 10-100 µg/ml bacteria; 100-1000 µg/ml yeasts up to 1.9 g/100 ml H2O
A2922 Colistin sulfate gram negative bacteria - non- proliferating germs too interaction with phospholipid components of the bacterial cell membrane; modifies permeability of the membrane and leads to an efflux of essential plasma substances 0.5-100 µg/ml > 1 mg/ml water-soluble
A0879 Cycloheximide (Actidion) fungi, eukaryotes binds to 80 S ribosome in eukaryotic cells; inhibits formation of peptide bond 10 µg/ml 10 mg/ml
A1943 D-Cycloserine gram positive, gram negative bacteria; various protozoa, spirochaeta, rickettsia competitive antagonist of D-alanine; Inhibition of the enzymes alanine-racemase, alanine-synthetase and several Transaminases; causes cell wall damages. 200 µg/ml 10-100 mg/ml in H2O or 0.1 M sodium phosphate buffer pH 8 (more stable at alkaline pH!); recommendation: prepare fresh immediately before use!
A2167 G418 disulfate toxic to bacteria, yeast higher plants, protozoa, mammalian cells Aminoglycoside Antibiotic; 50-1000 µg/ml 2 mg/ml in H2O or medium; adjust pH to 7.4; store at +4°C
(frequently 0.4-1 mg/ml)
A1492 Gentamycin sulfate gram positive/ negative germs inhibits protein synthesis by binding to the L6 protein of the 50 S ribosomal subunit 15-50 µg/ml 10-20 mg/ml in water, formamide
A5347 Hygromycin B Mycoplasma, eukaryotic and prokaryotic cells inhibits the protein synthesis by termination of the translocation and causes mistakes in transcription 10-400 µg/ml 400 mg/ml in H2O or buffer; store at -20°C
A2175 Hygromycin B - Solution     10-400 µg/ml ca. 41 mg/ml in H2O; store at -20°C
A1493 Kanamycin sulfate gram positive/ inhibits protein synthesis (translocation) 10-100 µg/ml 10 mg/ml in H2O
negative cocci & bacteria store at -20°C
A2190 Mitomycin C gram positive bacteria, some viruses, cytostatic covalent binding to DNA, cross-linking of complementary DNA strands; leads to strand breakes 10 µg/ml 0.2 mg/ml in PBS, sterilize by filtration; store up to 6 months at -20°C
A1894 Nalidixic acid gram negative cocci and bacteria inhibits specifically DNA replication in prokaryotes (DNA gyrase) 15 µg/ml CHCl3, Toluene,
5-10 mg/ml
in 0.1 N NaOH (pH 11)
A1944 Novobiocin sodium salt gram positive bacteria and cocci, gram negative cocci inhibition of initiation of DNA synthesis, competes with ATP binding at the DNA gyrase 1-5 µg/ml 100 mg/ml H2O (pH 7.5)
A3811 Nystatin dihydrate yeast, fungi Polyen Macrolide; interacts with sterols from cell membrane; changes permeability 20-50 µg/ml 100 mg/ml H2O, readily soluble in DMF and DMSO
A1837 Penicillin G potassium salt gram positive bacteria and cocci, gram negative cocci, Actinomycetes, Spirochaetae irreversible interaction with the transpeptidase; inhibits cell wall synthesis 50-100 µg/ml H2O
or 20-50 U/ml
A0890 Polymyxin B sulfate gram negative bacteria - non- proliferating germs too interaction with phospholipid components of the bact. cell membrane; changes permeability of the membrane and causes efflux of essential plasma compounds 50 µg/ml 25 mg/ml H2O, MeOH
A2856 Puromycin dihydrochloride gram positive bacteria; several Eukaryotes analogon of the terminal amino acyladenosine part of tyrosyl-tRNA; binds instead of amino acyl-tRNA to ribosomes; termination of protein biosynthesis; 10-100 µM 100 mM in H2O; store at -20°C
A2220 Rifampicin Prokaryotes, Eukaryotes (high dosis) inhibits RNA synthesis by binding to the b-subunit of the RNA polymerase 150 µg/ml 34 mg/ml in DMSO, MeOH
A3834 Spectinomycin · 2HCl · 5H2O aerobic gram-negative, -positive bacteria and mycoplasma species Aminoglycoside antibiotic; bacteriostatic; inhibits translocation of peptidyl tRNA from the A site to the P site 100 µg/ml 10 mg/ml in H2O, MeOH, DMSO
A1852 Streptomycin sulfate mycobacteria, gram negative/ positive cocci and bacteria inhibits protein synthesis by binding to the 30 S ribosomal subunit; causes missreading of the DNA code 10-100 µg/ml 10-50 mg/ml in H2O store at -20°C
A2228 Tetracyclin hydrochloride     12 µg/ml approx. 12 mg/ml in H2O
A2242 Tunicamycin gram positive bacteria, yeasts, fungi, viruses inhibits the GlcNAc-1P transferase (linking fatty acids : saccharides) 0.1-5 µg/ml 10 µg/ml in 0.05 M NaOH store at -20°C
A1839 Vancomycin hydrochloride bacteriostatic and bacterizide against gram positive cocci and bacteria amphoteric glyopeptide antibiotic; binds to bacterial cell wall precursors (peptidoglycans) 1-25 µg/ml soluble in water ›100 mg/ml