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Assays, Inhibitors & Supplements

A well-known inductor of protein expression in transgenic models is IPTG. We offer different qualities and achieve high customer satisfaction at good prices

A7211 IPTG from plant origin galactose
A4773 IPTG for molecular biology
A1008 IPTG BioChemica

One of the most important supplemental chemicals you need for Protein Biochemistry work is Albumin. Our customer favourites are:

A4344 Albumin crude from chicken egg
A2245 Albumin from chicken egg
A0850 Albumin (BSA) EIA and RIA grade
A2244 Albumin (BSA) Fraction V (pH 5.2)
A1391 Albumin (BSA) Fraction V (pH 7.0)
A6588 Albumin (BSA) Fraction V (pH 7.0) for Western blotting
A0849 Albumin (BSA) for microbiology

When working with proteins, proteindegradation and digestion is an issue. ITW Reagents offers an interesting range of products to impede this to happen. We also have products for basic protein assays and detection. One of our customer favorites is Coomassie® Brilliant Blue (A3480, A1092).


Protease Inhibitors - Survey

Prod.-No. Description Inhibitor Class Target Enzyme Recommended Working Concentration Stock Solution
A1421 AEBSF Serine proteases Thrombin, Chymotrypsin, Kallikrein, Plasmin, Proteinase K, Trypsin 0.1 - 2 mM 20 - 100 mM in buffer
A2266 epsilon-Aminocaproic acid Serine proteases   5 mM 500 mM in buffer
A2129 Antipain Serine/Cysteine proteases Trypsin, Papain, Cathepsin B 10 - 50 µg/ml 10 mg/ml in water, DMSO, MeOH
A2132 Aprotinin Serine proteases Trypsin, Chymotrypsin, Kallikrein, Plasmin 2 - 10 µg/ml 10 mg/ml in water
A2144 Chymostatin Serine/Cysteine proteases α-, β-, γ-, δ-Chymotrypsin, Papain, Cathepsin A, B and D 6 - 60 µg/ml 20 mg/ml in DMSO, acetic acid
(10 - 100 µM)
A2157 E-64 Cysteine proteases Papain, Bromelain, Calpain, Cathepsin B, H, L, Tumor-Cathepsin, Streptococcus-Protease, Ficin 10 µM 1 - 10 mM in DMSO, 50 % EtOH
A0878 EGTA Metallo proteases KEX 2, Calcium-dependent proteases 1 - 10 mM in aqueous solution
A1666 Iodoacetamide Serine proteases Cerastocytin 1 - 5 mM in aqueous solution
(185 - 925 µg/ml)
A2183 Leupeptin Serine/Cysteine proteases Plasmin, Trypsin, Papain, Cathepsin B, Thrombin, Calpain 5 - 50 µg/ml 1 mg/ml in aqueous solutions
(10 - 100 µM)
A2205 Pepstatin A acidic proteases, Aspartic proteases Pepsin, Cathepsin D, Renin, HIV- and MMTV-Protease 1 - 5 µM in MeOH, EtOH, acetic acid, DMSO
(0.7 - 3.5 µg/ml)
A0999 PMSF * Serine/Cysteine proteases Trypsin, Chymotrypsin, Thrombin, Papain 0.1 - 1 mM 10 - 100 mM in EtOH
(1.74 - 17.4 mg/ml)
A1799 TLCK Serine proteases Trypsin, Cerastocytin, not Chymotrypsin 50 µg/ml 1 - 20 mg/ml in buffer
pH <= 6

* The inhibition of cysteine proteases can be reverted by reducing thioles (e.g. DTT).

AEBSF (4-(2-Aminoethyl)-benzolsulfonylfluoride); APMSF (4-Amidinophenylmethanesulfonylfluoride); E-64 (N-(trans-Epoxysuccinyl)-L-leucine-4-guanidinobutylamide); EDTA (Ethylenediaminetetraacetate); EGTA (Ethyleneglycol-bis-(2-aminoethyl)-tetraacetate); EtOH (Ethanol); MeOH (Methanol)PMSF (Phenylmethanesulfonylfluoride); TLCK (Nα-Tosyl-L-lysine chloromethylketone).